AutoRun is a Windows feature that allows files or programs to immediately run as soon as a removable media device, such as a USB stick or CD-ROM, is connected to a computer.AutoRun feature could allow malicious code to spread. One of the vectors by which the infectious Conficker, or Downadup, worm propagates is through pen drives / other removable storage medias. you can manually delete/remove the autorun.inf file using following steps.
Step 1. First you Restart your computer to safe mode ! Press F8 to get the menu (while windows starting ).
Step 2. Open command prompt ( Go to Start > RUN and type CMD)
Go to the drive. ("cd e:" - use your drive letter )
Go to the root directory (cd \ )
then change the attributes of the autorun file
attrib -h -r -s autorun.inf
-h = remove Hidden property
-r = remove Read only property
-s = remove System file property
Now you delete the file
del -h -r -s autorun.inf
Note : Only open that drive after the restart. Done !
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